We Have comment the 61 oxygen so oxygen plays a major and vital role in this fermentation and here once the oxygen is not supplied then the fermentation process will not be done how let us enough.  this firstly why why this oxygen is used this position is used for the fast metabolism of the for the fast metabolism to obtain the product and once the oxygen content will get decreased or else if you put if you inject no amount of oxygen if you through the inlet then high oxygen demand will occur inside the fermentation tank.  which is shortly said as maturity and once this high oxygen demand will be done then the fermentation process will not be done so in this way the oxygen placed are major and vital role in this fermentation process.  so now let us see the steps ofthe fermentation. so enough let us seethe steps which are included in this fermentation so how the sub fermentation process will be done so firstly what you are going to do is that you are going to select a particular type of microorganism strains.  for example if you want to produce lactic acid you can take lactic acid bacteria right and if you want to produce citric acid . you can take Aspergillus Niger so it is where you are going to take this particular bacteria which produces particular type of organic acids so you are going to select a particular strains of the organism and you are going to inoculate them in the fermentation tank and now what you are going to do is that firstly the fermentation tank should be cleaned and this fermentation tank is called as fermenter and it is also called as bioreactor don’t get confused and now what you are going to do is that you are going to clean the fermentation with the water I have said you that the water plays a major and vital role to prevent the contamination of the microorganisms which are going to insertion this fermentation tank.  so you are going to wash it and after washing then you are going to you are going to add these microorganisms into this fermentation tank or as a bioreactor tank . and then along with these microorganisms you are also going to add the nutrient medium right as I have said to the nutrient medium Ingram’s water carbohydrates lipids and fats growth factors buffers and oxygen this oxygen lay some major and vital role don’t forget to supply the oxygen and his oxygen will be supplied through oxygen Inlet there is oxygen and LED so if you see the diagram then you can understand so this will be the diagram of a bioreactor tank or as a fermentation tank.  so if you see here this is the agitation system agitation system is nothing but it consists of a motor and inside it cuts off a blades so this blades plays a major and vital role to convert primary metabolites into the secondary metabolites and that primary metabolites will be he will be inserted into this fermentation tank.  by this pipe this is called as feeding pump and through this feeding pump you are going to add the primary metabolites and even you are going to add the nutrient medium.  here the medium box is present right so slowly this medium will be introduced into this into this fermentation tank along with these primary metabolites of your interest and now what you are going to do this is the oxygen Inlet.  I have said you write oxygen plays a major and vital role right so from here oxygen will beintroduced into this fermentation tank. and here it now this tank consists ofnutrient medium along with the primarymetabolites and now we are going toswitch on this fermentation tank.  suchthat the flat blades which are presentinside this tank starts rotating becauseof this motor which is present in theagitation system and now you are goingto control the temperature and alongwith the temperature you are also goingto control the pH and that we are goingto rotate these flat blades be the powerwhether by using this electricity andthen after a particular period of timethe primary metabolites will getconverted into the secondary metabolitesso enough that secondary metabolites will get produced out through thisoutlet and now we are going to switch onthe tab andwe’ll get switched on . and then this allof the secondary metabolites , which hasbeen produced will come out through thisoutlet and not this outlet you know youare going to take all of the cigarettemetaphor it’s from this outlet andyou’re going to purify them and you aregoing to filtrate them and once youfilled it and purified then the pureform of the secondary metabolite will beobtained so this is the this will be theabout the diagram of the bioreactor tank. so after the fermentation process whatwill happen.